Cornell University Department of Plant Breeding and Genetics

EDUCATION

Ph.D. in Molecular Entomology - University of Minnesota, 1988-1992
Insect Molecular Genetics, Advisor: Dr. Ann M. Fallon
Thesis: Gene Amplification in an Insect Cell Culture System. Studied the molecular processes of gene amplification in mosquito cells as a response to drug selection pressure. Developed novel selectable marker system for gene transformation in insect tissue culture cells.

M.S. in Entomology, Minor: Biochemistry, University of Nebraska-Lincoln, 1986-1988
Major Advisor: Dr. Z B Mayo
Thesis: Resistance in Schizaphis graminum (Homoptera: Aphididae) Induced by
Continuous Exposure to Systemic Insecticides. Studied the genetic and molecular mechanism responsible for organophosphate resistance in aphids.

B.S. in Agronomy and Integrated Pest Management, University of Nebraska-Lincoln, 1980-1984
Major emphasis: Crop Protection.
Minor: Chemistry/Biochemistry

PROFESSIONAL EXPERIENCE

CORNELL UNIVERSITY 2005 to present

Agricultural Biotechnology Support Project II (ABSP II) Director
Project director for ABSPII funded by the United States Agency for International Development (USAID) and led by Cornell University, CALS, International Programs.
• Provide overall management of the project.
• Develop product plans for South Asia, South East Asia; East, Central and West Africa.
• Develop communication outreach and technology transfer workshops.

SYNGENTA PLANT SCIENCE 2002 – 2004
Global Cotton Traits Technical Manager
Managed technical and product development activities leading to global commercial development of transgenic traits in cotton.
• Project leader for VipCotTM insect resistant cotton.
• Developed and managed technical working relationships with internal Syngenta Crop Protection business units and third party companies with the goal of commercializing trait-based products.
• Technical lead for all business and commercial negotiations involving cotton traits.
• Coordinated laboratory and field research.

SYNGENTA PLANT SCIENCE AND AGRIBUSINESS 2001 – 2002
Cotton Biotechnology Crop Leader
Research and technology project manager for all biotech traits for cotton biotech program.
• Project manager for VipCotTM insect resistant cotton.
• Established and managed third party collaborative research and testing agreements.
• As Potato Biotechnology Crop Leader – managed internal and third party biotechnology research program.



NOVARTIS AGRIBUSINESS BIOTECHNOLOGY RESEARCH INC. 1998 – 2001
Staff Scientist II
Project Leader: Conceived project ideas, initiated and developed projects with the intent of
commercialization of transgenic crop plants expressing recombinant insecticidal proteins.
• As Cotton Biotechnology Champion, introduced biotech traits into transgenic cotton.
• Developed and implemented research platform for transgenic cotton program including gene discovery, transgene design and vectoring, transformation and transgenic plant selection and characterization.
• Directed the entomology research team and managed several third party gene discovery research collaborative projects.
• Built an insecticidal protein discovery platform for sucking insects pests.
• As Vip3A Project leader, managed a team of five molecular biologists studying the biology and mode of action of the novel insecticidal protein.

ACADEMIC EXPERIENCE

UNIVERSITY OF WASHINGTON 1995 – 1998
Research Associate/Senior Research Fellow - Department of Medical Genetics
Studied gene transfer and heterologous gene expression in mammalian model systems.
• Primary focus of research was to develop efficient gene transfer technology for human
gene therapy applications.
• Conducted research to develop genetic insulator elements for use in vertebrate systems.
• Develop yeast spheroplast cell fusion technology for the transfer of YAC DNA into mammalian tissue culture cells.

UNIVERSITY OF WISCONSIN 1992 – 1995
Postdoctoral Research Associate - Department of Entomology
Conducted research to understand GABA receptor mediated cyclodiene resistance in insects.
• First to develop stable tissue culture system expressing functional GABA receptors.
• Worked toward the development of a novel selectable marker system for the genetic manipulation of Drosphila and mosquito species.

SOUTH CENTRAL RESEARCH AND EXTENSION CENTER, Clay Center, Nebraska 1985 – 1986
Research Technician - University of Nebraska
Designed and implemented herbicide field trials to evaluate efficacy of experimental and
registered herbicides.

ADDENDUM

THIRD PARTY MATERIAL TRANSFER AND RESEARCH AGREEMENTS

Discovery Research:
Diversa Corporation, San Diego, CA
University of Florida, Gainesville FL
Plant Research International B.V., Wageningen, Netherlands
Federal University of Ceara, Fortaleza, Brazil
University of Guelph , Ontario, Canada
International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
EMBRAPA, Brasilia, Brazil
CSIRO, Division of Entomology, Canberra, Australia

Cotton Transformation and Biotechnology:
Texas Tech University, Lubbock, TX
Institute of Molecular Agrobiology, Singapore
CSIRO, Division of Plant Industry, Canberra Australia

Vip Cotton Efficacy and Field Testing:
Delta and Pine Land Cotton Seed Co., Scott, MS
Emergent Genetics International, Inc./ Stoneville Pedigreed Seed, Memphis, TN
Bayer Cotton Seed International, Leland, MS
Phytogen/DAS, Woodland, CA
Vietnam Cotton Company, Ho Chi Minh City, Vietnam
Sichuan Agriculture Academy of Science, Jianyang, China
CSIRO, Division of Plant Science, Narrabri, Australia
Syngenta Seeds India, Aurangabad, India
INRA - Saenz Peña, Chaco, Argentina
Burkina Faso and Zimbabwe, Africa
Multiple USA Universities

Patent Applications
Three patent applications on novel Vegetative insecticidal proteins (Vip proteins).
Three patent applications on the insect resistant cotton events.

Recent International Meeting Presentations
5th Pacific Rim Conference on the Biotechnology of Bacillus thuringiensis and its Environmental Impact, Hanoi, Vietnam, Nov. 18-21, 2003
World Cotton Research Conference 3 – Cape Town, Republic of South Africa, March 9-13, 2003
Interactive Session on VipCot, New Delhi, India, Dec. 17, 2003

Academic Grants
Co-PI NIH RO1 - Cloning cyclodiene resistance from Aedes mosquitoes. ($470,335), 1994-1997
Co-PI NIH Program Project - A genetic approach to mosquito-borne disease control.
($3,750,078), 1995-2000

PUBLISHED ABSTRACTS AND PROCEEDINGS

Mi Kyong Lee, Frederick S. Walters, Hope Hart, Narendra Palekar, Jeng-Shong Chen and Frank Shotkoski. In Press. Vip3A: Vip: a novel insecticidal protein with broad spectrum lepidopteran activity. Proceedings of the 5th Pacific Rim Conference on Biotechnology of Bacillus thuringiensis and its environmental impact.

Shotkoski, F.A., V. Mascarenhas, R. Boykin & J-S. Chen. 2003. Vip: a novel insecticidal protein with broad spectrum lepidopteran activity. 2003 Proceedings of the Beltwide Cotton Conferences. p. 89

Shotkoski, F.A., A. Morris, A.A. James & R.H. ffrench-Constant. 1995. Analysis of the Rdl GABA receptor gene promoter in the Aedes aegypti mosquito. J. Cell. Biochem. 21A, 227.

Stillwell G., F. Shotkoski, A. Morris, A.A. James, and R.H. ffrench-Constant. 1994. Analysis of Rdl GABA receptor gene promoters in Drosophila and mosquitoes. Society for Neuroscience Abstracts. 20, 219.6.

Shotkoski, F.A., M. Thompson & R. ffrench-Constant. 1993. Conservation of resistance genes and mutations in cyclodiene resistant insects. Second International Symposium of Molecular Insect Science.

Fallon, A.M., F.A. Shotkoski & Y.-J. Park. 1992. Stable transfection of mosquito cells with a DNA construct containing the dihydrofolate reductase gene. In Vitro Cell & Dev Biol. 28, I-1012.

Shotkoski, F.A. & A.M. Fallon. 1990. Evidence for gene amplification in methotrexate-resistant mosquito cells. In Vitro Cell & Dev Biol. 26, 61A.

Refereed Publications

Shotkoski, FA, Jayachanadran G, Stamatoyannopoulos, Fallon AM. 1999. Sequence of a mosquito ribonucleotide reductase cDNA and evidence for gene amplification in hydroxyurea-resistant cells. Insect Mol Biol. 8, 565-70.

Neff T, Shotkoski F & Stamatoyannopoulos G. Stem cell gene therapy, position effects and chromatin insulators. 1997. Stem Cells. Suppl 1:265-71.

Dunkov, BC, Guzov VM, Mocelin G, Shotkoski F, Brun A, Amichot M, ffrench-Constant RH & Feyereisen, R. 1997. The Drosophila cytochrome P450 gene Cyp6a2: structure, localization, heterologous expression, and induction by phenobarbital. DNA Cell Biol. 16, 1345-56.

Coustau C, Carkion Y, Nappi A, Shotkoski F, ffrench-Constant R. 1996. Differential induction of antibacterial transcripts in Drosophila susceptible and resistant to parasitism by Leptopilina boulardi. Insect Mol Biol. 5, 167-72.

Coustau, C., Carkion, Y., Nappi, A., Shotkoski, F. & ffrench-Constant, R. 1996. Differential induction of antibacterial transcripts in Drosophila susceptible and resistant to parasitism by Leptopilina boulardi. Insect Mol. Biol. 5, 167-172.

Shotkoski, F., H.-G. Zhang, M.B. Jackson & R.H. ffrench-Constant. 1996. Stable expression of insect GABA receptors in insect cell lines: Promoters for efficient expression of Drosophila and mosquito Rdl GABA receptors in stably transformed mosquito cell lines. FEBS letters 380, 257-262.

Shotkoski, F.A., A.C. Morris, A.A. James & R.H. ffrench-Constant. 1996. Functional analysis of a mosquito ?-aminobutyric acid receptor gene promoter. Gene 168, 127-133.

Shotkoski, F., H.-J. Lee, H.-G. Zhang, M.B. Jackson & R.H. ffrench-Constant. 1994. Functional expression of insecticide resistant GABA receptors from the mosquito Aedes aegypti. Insect Molec. Biol. 3, 283-287.

ffrench-Constant, R., J.C. Steichen & F.A. Shotkoski. 1994. Polymerase chain reaction diagnostic for cyclodiene insecticide resistance in Aedes aegypti. Med. Vet. Entomol. 8, 99-100.

Shotkoski, F.A. & A.M. Fallon. 1994. Expression of an antisense dihydrofolate reductase transcript in transfected mosquito cells: Effects on growth and plating efficiency. Am. J. Trop. Med. Hyg. 50, 433-439.

Thompson, M, F.A. Shotkoski & R. ffrench-Constant. 1993. Cloning and sequencing of the cyclodiene resistance gene from the yellow fever mosquito Aedes aegypti: Conservation of the gene and resistance associated mutation with Drosophila. FEBS Letters, 325, 187-190.

Shotkoski, F.A. & A.M. Fallon. 1993. An amplified dihydrofolate reductase gene: Amplicon size and chromosomal distribution. Insect Molec. Biol. 2, 155-161.

Shotkoski, F.A. & A.M. Fallon. 1993. The mosquito dihydrofolate reductase gene functions as a dominant selectable marker in transfected cells. Insect Biochem Molec. Biol. 23, 883-893.

Fallon A.M., A. Gerenday, Q. Lan, C.A. Mazzacano, F.A. Shotkoski, CC. Wu, 1993. Transfection of mosquito cells: prospects for the embryo. In: Host regulated developmental mechanisms in vector arthropods. Vol. 3.

Shotkoski, F.A. & A.M. Fallon. 1991. An insect dihydrofolate reductase gene contains a single intron. Eur. J. Biochem. 201, 157-160.

Shotkoski, F.A. & A.M. Fallon. 1990. Genetic changes in methotrexate-resistant mosquito cells. Arch. Insect Biochem. Phys. 15, 79-92.

Shotkoski, F.A., Z B Mayo, & L.L. Peters. 1990. Induced disulfoton resistance in greenbugs (Homoptera: Aphididae). J. Econ. Entomol. 83, 2147-2152.

© 2006 Department of Plant Breeding and Genetics, Cornell University